| External factors: | Inauhzin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Lung cancer |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | INZ induced senescence in H460 or HCT116p53+/+, but not in HCT116p53?/?, cells, though to a much less degree than did Nutlin-3. |
| Target gene: | SIRT1 |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | SIRT1 |
| Target gene experiment: | Western blot |
| Target gene description: | INZ inhibited SIRT1 deacetylase activity in a dose‐dependent fashion and effectively inhibited this activity at 3?μM. This inhibition was specific to INZ and its chemical analogue INZ1 (methyl substituted R1), which activated p53 and decreased SIRT1 activity in a dose‐dependent fashion. |
| Regulatory pathway: | p53 |
| R-EF-Pathway: | Upregulation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | We found that INZ induced p53 level in a time-dependent manner as early as 6 h post-treatment in both p53-containing H460 and HCT116 cells. |
Annotation: