| External factors: | Glucocorticoid |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | By 48 and 72 h post-treatment, a significant increase in the percentage of SA-β-gal-positive cells was observed. |
| Target gene: | SIRT1 |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | SIRT1 |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | We found a small but significant reduction in RNA levels of sirt1, but a marked reduction in protein levels in glucocorticoid-treated cells compared with carrier-treated controls. |
| Regulatory pathway: | p53 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | In contrast, levels of acetylated p53 (but not phosphorylated p53) were higher in glucocorticoid-treated tenocytes compared with controls. While there were no significant differences in p53 RNA levels, RNA levels of p21cip/waf1, a pro-senescence modulator of p53 activity, were markedly elevated post-glucocorticoid treatment . |
Annotation: