| External factors: | Estrogen |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Breast cancer |
| Experiment: | SA-β-gal activity assay |
| Description: | The addition of 1 nM beta-estradiol to MCF-7 cells grown in charcoal-stripped serum brought the level of cellular senescence down to 35% β-galactosidase positive cells. |
| Target gene: | YPEL3 |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | YPEL3 |
| Target gene experiment: | SA-β-gal activity assay//RT-PCR//Knockdown |
| Target gene description: | In contrast, shLacZ expressing MCF-7 cells did show the expected induction of cellular senescence when grown in charcoal-stripped serum. Knockdown of YPEL3 expression was confirmed with relative mRNA expression. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: