| External factors: | Regulatory T cells |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | TRAP assay//Flow cytometry//SA-β-gal activity assay |
| Description: | Using the TRAP assay, we found that both nTregs and iTregs could induce the inhibition of telomerase activity in all the target cells.Upon co-cultivation with Tregs, we observed a significant decrease in the number of target cells in the S and G2/M phases and a reliable increase in the number of cells in the G0/G1 phases 30 days after transfection. β–Gal expression levels remained stably high over the days 60. β–Gal enzymatic activity also increased 45 days after transfection and remained high on day 60. |
| Target gene: | α+β+HTERT |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | α+β+HTERT |
| Target gene experiment: | Western blot//RT-PCR |
| Target gene description: | Western blotting results showed a significant decrease in full-length α+ β+ hTERT and an increase in α+β– hTERT and EndoG in the target cells co-cultured with nTregs or iTregs Real-time RT-PCR revealed that cultivation of the target cells in the absence of iTregs resulted in a gradual increase in α+ β+ hTERT expression, decrease in α+ β– hTERT expression and decrease in EndoG expression to the levels of the control target cells over 48 h. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: