Aging Overview

External factors

External factors: Regulatory T cells
Aging type: Accelerate
Aging characteristic:
Category: Other
Phenotype: Aging
Experimental category: L
Tissue type: Blood
Cell name: CD4+CD25?T,CD8+T,B,NK
PMID: 30025223
Experiment: TRAP assay//Flow cytometry//SA-β-gal activity assay
Description: Using the TRAP assay, we found that both nTregs and iTregs could induce the inhibition of telomerase activity in all the target cells.Upon co-cultivation with Tregs, we observed a significant decrease in the number of target cells in the S and G2/M phases and a reliable increase in the number of cells in the G0/G1 phases 30 days after transfection. β–Gal expression levels remained stably high over the days 60. β–Gal enzymatic activity also increased 45 days after transfection and remained high on day 60.


Regulatory relationship

Target gene: α+β+HTERT
R-EF-Target gene: Upregulation
Official symbol(s): α+β+HTERT
Target gene experiment: Western blot//RT-PCR
Target gene description: Western blotting results showed a significant decrease in full-length α+ β+ hTERT and an increase in α+β– hTERT and EndoG in the target cells co-cultured with nTregs or iTregs Real-time RT-PCR revealed that cultivation of the target cells in the absence of iTregs resulted in a gradual increase in α+ β+ hTERT expression, decrease in α+ β– hTERT expression and decrease in EndoG expression to the levels of the control target cells over 48 h.

Regulatory pathway: --
R-EF-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.