Aging Overview

External factors

External factors: HIV Tat
Aging type: Accelerate
Aging characteristic:
Category: Other
Phenotype: Aging
Experimental category: L
Tissue type: --
Cell name: PT-ECFC
PMID: 28236278
Experiment: Western blot
Description: Expression levels of GFAP(astrocyte activation as an indicator of aging) were significantly increased with aging in the both the WT and Tg rats.


Regulatory relationship

Target gene: SIRT1//MIR-34A//MIR-138
R-EF-Target gene: Downregulation//Upregulation//Upregulation
Official symbol(s): SIRT1//MIR-34A//MIR-138
Target gene experiment: Western blot//qRT-PCR
Target gene description: A significant decrease in SIRT1 protein content was detected in both A172 and human primary astrocytes following stimulation with HIV Tat;And in keeping with the in vivo data, there was increased expression of miRs-34a & -138 in the both cell line and primary astrocytes treated with HIV Tat compared with untreated control cells.

Regulatory pathway: NF-κB
R-EF-Pathway: Upregulation
Official symbol(s): NFKB1
Pathway experiment: Western blot
Pathway description: Pretreatment of A172 cells with Ikk-2 inhibitor SC514 (5 μM) significantly decreased HIV Tat-mediated induction of GFAP.This was further confirmed by the fact that transfection of A172 cells with mutant IκB resulted in amelioration of HIV Tat-mediated induction of GFAP .


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view