| External factors: | Baker ethanol extract |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | MTT assay//SA-β-gal activity assay//RT-PCR//Western blot |
| Description: | H2O2 exposure reduced cell proliferation compared to the normal cells;however,KPE treatment significantly reinstated the proliferative activity of Hs68 cells to almost the normal level .H2O2 exposure caused almost a 1.39-fold increase in the SA-β-gal activity in Hs68 cells, but this activity was dose-dependently decreased by KPE. The mRNA levels of cell-cycle inhibitors were significantly reduced by KPE treatment. The protein expression of cell-cycle inhibitors was dosedependently reduced upon KPE treatment .H2O2-induced senescent Hs68 cells exhibited higher IL-6 and IL-8 mRNA levels than that in normal cells; however, KPE treatment markedly reduced these mRNA levels.The protein expression of other inflammatory markers, NF-kB and COX-2, was also decreased by KPE treatment as compared to that in the H2O2 control . |
| Target gene: | SIRT1//PGC-1α |
| R-EF-Target gene: | Upregulation//Upregulation |
| Official symbol(s): | SIRT1//PPARGC1A |
| Target gene experiment: | RT-PCR//Western blot |
| Target gene description: | SIRT1 mRNA expression was significantly upregulated by KPE compared to that in the H2O2 control. The protein level of SIRT1 was also increased in KPE treated Hs68 cells. Reduced expression of PGC-1α, which is responsible for mitochondrial function and biogenesis, was recovered by KPE treatment. |
| Regulatory pathway: | PI3K-Akt//p53-p21//p16-pRb |
| R-EF-Pathway: | Downregulation//Downregulation//Downregulation |
| Official symbol(s): | PIK3CB-AKT//TP53-CDKN1A// |
| Pathway experiment: | RT-PCR//Western blot |
| Pathway description: | The H2O2-induced expression of PI3K and phospho-AKT was decreased by KPE without any visible changes in the total AKT level compared to that in the H2O2 control.Compared to young mice intrin- sically MA mice showed increased mRNA and protein levels of cell-cycle inhibitors, including p53, p21,p16, and pRb. In the KPE administered group, the p53, p21,p16, and pRb levels exhibited 33.1%, 44.4%, 40.8%, and 37 .4% reduction, respectively, compared to those in the intrinsically MA group. The protein levels of cell-cycle inhibi- tors were also attenuated by KPE treatment. |
Annotation: