| External factors: | Resveratrol |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Immunofluorescence |
| Description: | The number of SA-β-gal positive senescent cells was significantly increased in resveratrol-treated cells compared to control or DMSO treated cells. Furthermore, the percentage of SA-β-gal positive cells increased with the concentrations of resveratrol indicates that resveratrol induces premature senescence in a dose-dependent manner.Results showed that H3K9-me3 positive stained cells were also increased in BJ cells treated with the increasing concentrations of resveratrol. |
| Target gene: | SIRT1//SIRT2//P16 |
| R-EF-Target gene: | Downregulation//Downregulation//Activation |
| Official symbol(s): | SIRT1//SIRT2//P16 |
| Target gene experiment: | Western blot//qRT-PCR |
| Target gene description: | Interestingly, Western blotting analysis showed that expression of SIRT1 and SIRT2 proteins were significantly decreased upon 10 μM resveratrol treatment and also continued at higher concentrations (25, 50 and 100 μM). We confirmed these data by RT-qPCR analysis and showed that mRNA level of SIRT1 and SIRT2 was also significantly decreased starting with 10μM resveratrol treatment in BJ fibroblasts.As shown by Western blotting the expression levels of p53, p21CIP1 and p16INK4A were significantly increased upon 10 μM of resveratrol treatment in BJ cells, compared to control or DMSO. |
| Regulatory pathway: | p53-p21 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot |
| Pathway description: | As shown by Western blotting the expression levels of p53, p21CIP1 and p16INK4A were significantly increased upon 10 μM of resveratrol treatment in BJ cells, compared to control or DMSO . |
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