Aging Overview

External factors

External factors: Doxorubicin
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Aging
Experimental category: L
Tissue type: --
Cell name: BJ
PMID: 25924011
Experiment: SA-β-gal activity assay
Description: Thus in order to induce senescence we treated BJ cells with 50 and 100 ng/ml of doxorubicin for 5 days as suggested in literature [38]. Induction of senescence was evident with increased SA-β-gal activity, increased levels of p53 and p21CIP1 and γ-H2A.X foci formation.


Regulatory relationship

Target gene: SIRT1//SIRT3
R-EF-Target gene: Downregulation//Downregulation
Official symbol(s): SIRT1//SIRT3
Target gene experiment: Western blot
Target gene description: Remarkably WB analysis showed that expressions of SIRT1/2 were also slightly reduced during doxorubicin induced senescence.

Regulatory pathway: p53-p21
R-EF-Pathway: Activation
Official symbol(s): TP53-CDKN1A
Pathway experiment: SA-β-gal activity assay//Western blot
Pathway description: Additionally, when we tested p16 INK4A levels we found rather minor increase in p16INK4A levels suggesting doxorubicin induced senescence is mediated mainly by activation of p53-p21 pathway .


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view