External factors: | 5-AZA |
Aging type: | Prevent |
Aging characteristic: |
Category: | Chemical compounds |
Phenotype: | Aging |
Experiment: | Cell proliferation assay//SA-β-gal activity assay//Flow cytometry//qRT-PCR |
Description: | Cell activity in control culture declined after 24 hrs and began to increase after 48 hr till the last day of the experiment. Proliferation of cells cultured with 5-AZA was characterized by exponential cell growth during the whole experiment.we observed that 5-AZA treatment reduced the amount of enlarged cells in culture.Obtained results showed that the percentage of dead cells was decreased in 5-AZA treated cells (P < 0.01). Senescence associated accumulation of βgalactosidase (β-gal) was stained blue on histology samples. Number of positive stained cells was decreased in 5-AZA in comparison to control group.Quantitative analysis of transcripts revealed that expression of and p53 mRNA was significantly decreased in 5-AZA cultures . Similarly, the level of p21 transcript was also decreased in experimental cultures. The ratio of Bcl-2/BAX mRNA was increased (P < 0.01) after 5-AZA treatment. GAPDH was used as an endogenous control. |
Regulatory pathway: | -- |
R-EF-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation: