Aging Overview

External factors

External factors: 5-AZA
Aging type: Prevent
Aging characteristic:
Category: Chemical compounds
Phenotype: Aging
Experimental category: L
Tissue type: Adipose
Cell name: ASC
PMID: 27998022
Experiment: Cell proliferation assay//SA-β-gal activity assay//Flow cytometry//qRT-PCR
Description: Cell activity in control culture declined after 24 hrs and began to increase after 48 hr till the last day of the experiment. Proliferation of cells cultured with 5-AZA was characterized by exponential cell growth during the whole experiment.we observed that 5-AZA treatment reduced the amount of enlarged cells in culture.Obtained results showed that the percentage of dead cells was decreased in 5-AZA treated cells (P < 0.01). Senescence associated accumulation of βgalactosidase (β-gal) was stained blue on histology samples. Number of positive stained cells was decreased in 5-AZA in comparison to control group.Quantitative analysis of transcripts revealed that expression of and p53 mRNA was significantly decreased in 5-AZA cultures . Similarly, the level of p21 transcript was also decreased in experimental cultures. The ratio of Bcl-2/BAX mRNA was increased (P < 0.01) after 5-AZA treatment. GAPDH was used as an endogenous control.


Regulatory pathway: --
R-EF-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.