| External factors: | Metformin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | Exposure of MEFs to metformin as a single agent augmented baseline senescence measured as the percentage of cells that have SA-β-gal activity between approximately 3 4-fold. |
| Target gene: | MIR-200A |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | MIR-200A |
| Target gene experiment: | qRT-PCR |
| Target gene description: | When a 2-fold or greater difference in miRNA expression levels was used as the cut-off to determine significant regulatory effects on miRNAs involved in the EMT/ MET genetic program, we found that miR-200a (more than 20-fold), miR-141 and miR-429, three members of the miRNA200 family, were markedly upregulated in HDFs that have undergone SIS in response to metformin compared with young and old BJ-1 untreated fibroblasts . |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: