| External factors: | PQQ |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | The results showed that the percentage of cells stained by X?gal following 9 J/cm2 UVA irradiation was markedly increased compared with that in the control group (53 and 8%, respectively; P<0.05), while 50 ng/ml PQQ attenuated the ratio of positive staining compared with that of the UVA-only cells (29 vs. 53%, respectively; P<0.01) . |
| Target gene: | SIRT1//SIRT6 |
| R-EF-Target gene: | Upregulation//Upregulation |
| Official symbol(s): | SIRT1//SIRT6 |
| Target gene experiment: | Western blot//qRT-PCR |
| Target gene description: | At 72 h following the addition of 50 ng/ml PQQ to the culture media of UVA-irradiated HDFs, mRNA and protein expression levels of SIRT1 and SIRT6 were found to be increased . |
| Regulatory pathway: | Nrf2-HO-1 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | NFE2L2-HMOX1 |
| Pathway experiment: | Western blot//qRT-PCR |
| Pathway description: | As SIRT1/Nrf2/HO?1 is a classical anti?apoptotic pathway, the present study investigated whether this pathway was involved in UVA-induced cell apoptosis. Altered expression levels of Nrf2 and HO?1 mRNA expression levels were not found to be significant in HDFs pre-treated with 50 ng/ml PQQ without UVA irradiation. However, following UVA irradiation, PQQ was shown to alter the mRNA and protein expression of Nrf2 and HO?1. |
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