Aging Overview

External factors

External factors: Calorie restriction
Aging type: Prevent
Aging characteristic:
Category: Other
Phenotype: Aging
Experimental category: L
Tissue type: Kidney
Cell name: --
PMID: 26940461
Experiment: SA-β-gal activity assay//ELISA
Description: SA-β-gal assay is an effective method to measure cell senescence21and significantly fewer SA-β-gal positive cells were observed in CR conditions.Compared with AL group, reduced production of renal TNF-a and IL-1β were observed in mice subjected to CR ,indicated that CR can reduce renal inflammatory activity effectively.


Regulatory relationship

Target gene: SIRT6
R-EF-Target gene: Upregulation
Official symbol(s): SIRT6
Target gene experiment: Immunostaining//Western blot//SA-β-gal activity assay
Target gene description: Immunohistochemistry and Western blot analysis of SIRT6 indicated enhanced SIRT6 expression in CR condition. Our results strongly supports the evidence that CR can effectively prevent age-dependent renal failure and promotes in vivo SIRT6 expression.Using SA-β-gal staining, reduced number of SA-β-gal positive cells were observed in WI38 with SIRT6-WT overexpression, but not in cells with SIRT6-HY overexpression.

Regulatory pathway: NF-κB
R-EF-Pathway: Downregulation
Official symbol(s): NFKB1
Pathway experiment: DAPI staining
Pathway description: SIRT6 knockdown induced the translocation of NF-kB p65 into the nucleus along with enlarged cell bodies, indicated an activation of the NF-kB signaling and accelerated cell senescence .


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view