External factors: | Calorie restriction |
Aging type: | Prevent |
Aging characteristic: |
Category: | Other |
Phenotype: | Aging |
Experiment: | SA-β-gal activity assay//ELISA |
Description: | SA-β-gal assay is an effective method to measure cell senescence21and significantly fewer SA-β-gal positive cells were observed in CR conditions.Compared with AL group, reduced production of renal TNF-a and IL-1β were observed in mice subjected to CR ,indicated that CR can reduce renal inflammatory activity effectively. |
Target gene: | SIRT6 |
R-EF-Target gene: | Upregulation |
Official symbol(s): | SIRT6 |
Target gene experiment: | Immunostaining//Western blot//SA-β-gal activity assay |
Target gene description: | Immunohistochemistry and Western blot analysis of SIRT6 indicated enhanced SIRT6 expression in CR condition. Our results strongly supports the evidence that CR can effectively prevent age-dependent renal failure and promotes in vivo SIRT6 expression.Using SA-β-gal staining, reduced number of SA-β-gal positive cells were observed in WI38 with SIRT6-WT overexpression, but not in cells with SIRT6-HY overexpression. |
Regulatory pathway: | NF-κB |
R-EF-Pathway: | Downregulation |
Official symbol(s): | NFKB1 |
Pathway experiment: | DAPI staining |
Pathway description: | SIRT6 knockdown induced the translocation of NF-kB p65 into the nucleus along with enlarged cell bodies, indicated an activation of the NF-kB signaling and accelerated cell senescence . |
Annotation: