| External factors: | Vitamin D3 |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SDS-PAGE//GO analysis |
| Description: | We found that in aged worms, D3 treatment significantly decreased the number of detectable and identified SDS-insoluble proteins compared to control samples .Previous work found that reducing expression of several genes encoding proteins suppressed by D3 treatment in aged worms by RNAi resulted in significant lifespan extension. |
| Target gene: | SKN-1 |
| R-EF-Target gene: | -- |
| Official symbol(s): | POU2F3 |
| Target gene experiment: | Lifespan assay |
| Target gene description: | We observed no lifespan extension by D3 for skn-1(zu135) mutant worms, demonstrating that SKN-1 is requiredfor the effects of D3 feeding. |
| Regulatory pathway: | IRE-1-XBP-1 |
| R-EF-Pathway: | -- |
| Official symbol(s): | XBP1 |
| Pathway experiment: | Lifespan assay |
| Pathway description: | We found that the D3-induced increase on survival was dependent on IRE-1/XBP-1 signaling. Worms carrying the loss-of-function allele,ire-1(v33), showed significantly reduced lifespan with D3 feeding compared to vehicle-treated worms . Lifespan of worms maintaining the loss-of-function allele, xbp-1(zc12), showed no significant change with D3 feeding compared to vehicle-treated worms. Interestingly, ire-1(v33) mutant worms exhibited a shortened lifespan upon D3 feeding. |
Annotation: