Aging Overview

External factors

External factors: G-quadruplex interActivate agent S2T1-6OTD
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Tumor
Experimental category: L
Tissue type: --
Cell name: D341,D425,Daoy ,BT-12,BT-16
PMID: 20053783
Experiment: Telomere length assay//SA-β-gal activity assay//Cell morphological analysis
Description: Treatment with S2T1-6OTD at 0.04 μmol/L resulted in telomere shortening in all MB and AT/RT cells tested.The morphologic examination of the 0.04 μmol/L S2T1- 6OTD–treated cells at the plateau phase showed an increased proportion of flat and giant cells with phenotypic characteristics of senescence and positive for senescence-associated β-galactosidase.


Regulatory relationship

Target gene: C-MYC//HTERT
R-EF-Target gene: Downregulation//Downregulation
Official symbol(s): C-MYC//HTERT
Target gene experiment: qRT-RCR//ELISA
Target gene description: Treatment with S2T1-6OTD resulted in significant reductions of c-Myc mRNA and protein expression in all other MB and AT/RT cell lines tested .it was found that S2T1-6OTD could indeed reduce hTERT mRNA expression in BT-12, BT-16, DAOY ,and D341 cells.S2T1-6OTD reduced telomerase activity in all cell lines tested, with maximum inhibition in D341 (64%) and a minimum 17% in D425.



Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.