External factors: | G-quadruplex interActivate agent S2T1-6OTD |
Aging type: | Accelerate |
Aging characteristic: |
Category: | Chemical compounds |
Phenotype: | Tumor |
Experiment: | Telomere length assay//SA-β-gal activity assay//Cell morphological analysis |
Description: | Treatment with S2T1-6OTD at 0.04 μmol/L resulted in telomere shortening in all MB and AT/RT cells tested.The morphologic examination of the 0.04 μmol/L S2T1- 6OTD–treated cells at the plateau phase showed an increased proportion of flat and giant cells with phenotypic characteristics of senescence and positive for senescence-associated β-galactosidase. |
Target gene: | C-MYC//HTERT |
R-EF-Target gene: | Downregulation//Downregulation |
Official symbol(s): | C-MYC//HTERT |
Target gene experiment: | qRT-RCR//ELISA |
Target gene description: | Treatment with S2T1-6OTD resulted in significant reductions of c-Myc mRNA and protein expression in all other MB and AT/RT cell lines tested .it was found that S2T1-6OTD could indeed reduce hTERT mRNA expression in BT-12, BT-16, DAOY ,and D341 cells.S2T1-6OTD reduced telomerase activity in all cell lines tested, with maximum inhibition in D341 (64%) and a minimum 17% in D425. |
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