| External factors: | Cigarette smoke extract |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Chronic obstructive pulmonary disease |
| Experiment: | Flow cytometry//Colony formation assay//SA-β-gal activity assay//Western blot |
| Description: | CSE reduced, in a dose dependent manner, short-term cell proliferation;CSE increased beta galactosidase staining;CSE increased p21 expression. |
| Target gene: | SURVIVIN |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | SURVIVIN |
| Target gene experiment: | Flow cytometry |
| Target gene description: | CSE increased, in a dose dependent manner, survivin expression (increase versus baseline of CSE 10% = 13 ± 10; p b 0.01; increase versus baseline of CSE 20% = 26 ± 16; p=0.002). |
| Regulatory pathway: | SIRT1-FOXO3 |
| R-EF-Pathway: | Downregulation |
| Official symbol(s): | SIRT1-FOXO3 |
| Pathway experiment: | Western blot |
| Pathway description: | CSE in a dose dependent manner reduced nuclear expression of SIRT1; CSE reduced nuclear expression of FoxO3, in a dose dependent manner. |
Annotation: