| External factors: | Carbocysteine |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Chronic obstructive pulmonary disease |
| Experiment: | Flow cytometry//Colony formation assay//SA-β-gal activity assay//Western blot |
| Description: | Incubation with CARB significantly counteracted this phenomenon in cells exposed to CSE; CSE increased beta galactosidase staining and incubation with CARB completely reverted this phenomenon;CSE increased p21 expression and CARB counteracted this phenomenon. |
| Target gene: | SURVIVIN |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | SURVIVIN |
| Target gene experiment: | Flow cytometry |
| Target gene description: | A dose dependent manner, survivin expression and the costimulation with CARB significantly counteracted this phenomenon (reduction versus CSE 10% =17 ± 13; p b 0.02 reduction versus CSE 20% = 16 ± 11; p b 0.007). |
| Regulatory pathway: | SIRT1-FOXO3 |
| R-EF-Pathway: | Upregulation |
| Official symbol(s): | SIRT1-FOXO3 |
| Pathway experiment: | Western blot |
| Pathway description: | CSE in a dose dependent manner reduced nuclear expression of SIRT1; CSE reduced nuclear expression of FoxO3, in a dose dependent manner. |
Annotation: