| External factors: | AngII |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | Ang II induced a dose-dependent increase in the fraction of cells staining positive for β-galactosidase, indicating an increased fraction of senescent cells. |
| Target gene: | TERT//UCP2//P-AKT//C-MYC//P53 |
| R-EF-Target gene: | Upregulation//Upregulation//Upregulation//Upregulation//Upregulation |
| Official symbol(s): | TERT//UCP2//P-AKT//C-MYC//P53 |
| Target gene experiment: | Western blot |
| Target gene description: | Incubation with Ang II further elevated TERT protein levels from 0.006±0.041 at baseline, to 0.480±00.031 in the presence of 10 lM Ang II, UCP2 protein levels from 0.297±0.051 to 2.512±0.024, p-Akt protein levels from 0.012± 0.024 to 0.874± 0.015, c-myc protein levels from 0.521 ±0.015 to 1.064± 0.025, and p53 protein levels from 0.035± 0.047 to 1.195 ±0.029 but Akt levels were not significantly affected by incubation with even 10 lM Ang II. |
| Regulatory pathway: | PI3K-Akt |
| R-EF-Pathway: | -- |
| Official symbol(s): | PIK3CB-AKT |
| Pathway experiment: | SA-β-gal activity assay |
| Pathway description: | β-Galactosidase staining indicated a decline in the fraction of senescent cells with increasing LY concentration. |
Annotation: