External factors: | Telomestatin |
Aging type: | Accelerate |
Aging characteristic: |
Category: | Chemical compounds |
Phenotype: | Fibrosarcoma |
Experiment: | Immunostaining |
Description: | Treatment of HT1080 cells with 1, 2, and 5μM telomestatin for 48 h induces a dose-dependent decrease of the G-overhang signal, which represents 32, 15, and 10% of the untreated control, respectively.Telomestatin treatment induces a marked DNA damage response evidenced by a strong increase in theγH2AX foci.Interestingly, the exposure of HT1080 cells to telomestatin (2μM)induces a rapid telomere shortening detectable after short term treatment. |
Target gene: | POT1//TRF2 |
R-EF-Target gene: | Downregulation//Downregulation |
Official symbol(s): | POT1//TERF2 |
Target gene experiment: | Immunostaining |
Target gene description: | Telomestatin treatment of HT1080 cells(2μM,48 h) induced a noticeable decrease of the TRF2 signal at telomeres .Telomestatin strongly reduced the GFP-POT1 punctated signal associated with telomeres to nearly undetectable levels, as compared with untreated controls.The dose-dependent effect of telomestatin was also studied in HT1080GFP-POT1 cells after 48 h of treatment. |
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