Aging Overview

External factors

External factors: Telomestatin
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Fibrosarcoma
Experimental category: L
Tissue type: --
Cell name: HT-1080
PMID: 17050546
Experiment: Immunostaining
Description: Treatment of HT1080 cells with 1, 2, and 5μM telomestatin for 48 h induces a dose-dependent decrease of the G-overhang signal, which represents 32, 15, and 10% of the untreated control, respectively.Telomestatin treatment induces a marked DNA damage response evidenced by a strong increase in theγH2AX foci.Interestingly, the exposure of HT1080 cells to telomestatin (2μM)induces a rapid telomere shortening detectable after short term treatment.


Regulatory relationship

Target gene: POT1//TRF2
R-EF-Target gene: Downregulation//Downregulation
Official symbol(s): POT1//TERF2
Target gene experiment: Immunostaining
Target gene description: Telomestatin treatment of HT1080 cells(2μM,48 h) induced a noticeable decrease of the TRF2 signal at telomeres .Telomestatin strongly reduced the GFP-POT1 punctated signal associated with telomeres to nearly undetectable levels, as compared with untreated controls.The dose-dependent effect of telomestatin was also studied in HT1080GFP-POT1 cells after 48 h of treatment.



Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.