| External factors: | Ang II |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Cerebrovascular aging |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | Two different concentrations of AngII (10-7 M and 10-6 M) incubation for 5 days caused significant positive β-gal staining (blue) in cultured hBVSMCs,Two concentrations of AngII incubation also triggered NBS1 protein(molecular marker of senescence)level in hBVSMCs. |
| Target gene: | TRF2 |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | TERF2 |
| Target gene experiment: | Western blot |
| Target gene description: | AngII caused significant downregulation of TRF2. |
| Regulatory pathway: | p53//Siah-1 |
| R-EF-Pathway: | Upregulation//Upregulation |
| Official symbol(s): | TP53//SIAH1 |
| Pathway experiment: | Western blot |
| Pathway description: | AngII treatment for 5 days enhanced total p53 protein expression by ~4.5 folds in hBVSMCs as well as the phospho-p53 expression.FGF21 inhibited Siah-1 expression induced by AngII. |
Annotation: