| External factors: | Acrolein |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Cell counting//Cell viability assay |
| Description: | Repetitive acrolein exposure reduced cell viability in a dose-dependent manner.During that time period, cell growth was significantly reduced in cells previously exposed to acrolein Cells previously exposed to acrolein exhibited a significant increase in SA β-gal activity compared with control cells. |
| Target gene: | WRN |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | WRN |
| Target gene experiment: | Western blot |
| Target gene description: | In HFL-1 cells exposed to 25 μM acrolein, steadystate levels of WRN were reduced at 48 hr (p = 0.07) and significantly reduced at 72 hr . |
| Regulatory pathway: | p53-p21 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot//SA-β-gal activity assay//Cell counting |
| Pathway description: | Although both p53 and p21 levels were higher in acrolein-exposed cells than in controls during the period, they appeared to be slightly lower on day 3. In addition, both p53 and p21 levels did increase in control cells over the 3-day period . Suppression of p53 prevented acrolein-induced growth inhibition. Consistent with the cell growth data, p53 knockdown significantly attenuated SA β-gal activity in acrolein-exposed cells. |
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