| External factors: | lipopolysaccharide |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Flow cytometry//SA-β-gal activity assay//Cell morphological analysis |
| Description: | A senescence-like morphology was observed after repeated stimulation with LPS for 3 or 6 times. The morphology of DPSCs was characterized by a flat shape and increased size.Flow cytometry showed that more DPSCs were restricted to the G1 phase harvested from DPSCs treated with LPS 3 times and 6 times (3 times: 62.46 %±4.6 %, 6 times:66.80 %±4.8 %) than in the DPSCs from the control group or DPSCs treated with LPS only once (control: 45.09 %±3.6 %,once: 46.23 %±3.9 %;).After treatment with LPS, the number of SA-β-gal–positive cells clearly increased in LPS-treated DPSCs, with the strongest staining being seen in DPSCs receiving LPS stimulation 6 times. |
| Target gene: | γ-H2A.X//P16 |
| R-EF-Target gene: | Upregulation//Upregulation |
| Official symbol(s): | H2AX//P16 |
| Target gene experiment: | Western blot//RT-PCR//qRT-PCR |
| Target gene description: | The result suggested that the stimulation of LPS 3 or 6 times markedly increased γ-H2A.X protein expression. Analysis by reverse transcription plus PCR (RT-PCR) showed that levels of γ-H2A.X were up-regulated after exposure to LPS 3 or 6 times.After stimulation with LPS, the expression level of p16INK4A was up-regulated in a manner dependent on the number of repeated treatments. |
| Regulatory pathway: | TLR4 |
| R-EF-Pathway: | Upregulation |
| Official symbol(s): | TLR4 |
| Pathway experiment: | Western blot |
| Pathway description: | After stimulation with LPS, the expression level of TLR4 was upregulated in a manner dependent on the number of repeated treatments . |
Annotation: