Aging Overview

Aging gene

Gene name: STING1
Aging type: Accelerate
Aging characteristic:
Tissue type: --
Cell name: Chondrocytes
Gene ID: 340061
Category: protein coding
Phenotype: Osteoarthritis
Experimental category: L
PMID: 33414452
Experiment: SA-β-gal activity assay//Western blot
Description: The results showed higher p16INK4a and p21 protein mea- sures in the STING-overexpressed and IL-1β-treated chondrocytes. Although STING knockdown significantly reduced the IL-1β-simulated upregulation of p16INK4a and p21 proteins, the knockdown of STING alone did not affect these proteins expression levels. We established that si-P65 remarkably reduced the expression of STING-induced senescence-associated (p16INK4a and p21), and apoptosis-associated (BAX, Cyto-c, as well as cleaved- caspase-3) proteins and increased the expression of BCL-2. The SA-β-gal and TUNEL staining confirmed that STING regulates senescence and apopto- sis via the NF-κB-signaling axis activation.


Regulatory relationship

Regulatory pathway: NF-κB
R-AG-Pathway: Activation
Official symbol(s): NFKB1
Pathway experiment: Western blot//Immunofluorescence//SA-β-gal activity assay//TUNEL assay
Pathway description: The results showed that higher phosphorylation of P65 and iκb protein levels were seen in STING overexpressing and IL-1β treated chondrocytes.The analysis of the immunofluorescence of nuclear P65 confirmed that STING activates NF-κB signaling.STING mediates the degradation of the ECM degradation through the NF-κBsignaling cascade.STING regulates senescence and apoptosis via the NF-κB-signaling axis activation.Si-P65 remarkably reduced the expression of STING-induced senescence-associated (p16INK4a and p21),and apoptosis-associated (BAX,Cyto-c,as well as cleavedcaspase-3) proteins and increased the expression of BCL-2. The SA-β-gal and TUNEL staining confirmed that STING regulates senescence and apoptosis via the NF-κB-signaling axis activation.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

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