| Gene name: | ASIC3 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | NP-MSCs |
| Experiment: | Western blot//CCK-8 assay//Flow cytometry//SA-β-gal activity assay//ELISA |
| Description: | ASIC1 and ASIC3 were progressively upregulated in NP-MSCs derived from mild,moderate through to severely degenerated IVDs. CCK-8 showed that Pfirrmann grade II and grade IV isolates failed to grow at pH 6.6 in contrast to normal culture conditions.proliferative cells (S+G2/M phases) substantially declined from 25.72 to 7.12%.Acidic culture resulted in a time dependent increase in the percentage of senescence cells,reaching a striking ~80% of cells after 5 days.NP-MSC increased levels of secreted IL-6 and IL-8 by NP-MSCs under pH 6.6 culture conditions. |
| Target gene: | P53//P21//P27//RB1//P16//MMP3//MMP9 |
| Official symbol(s): | P53//P21//P27//RB1//P16//MMP3//MMP9 |
| R-AG-Target gene: | Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Exposure of isolated NP-MSCs to acidic growth conditions resulted in relatively increased levels of p53,p21,p27,Rb1 and p16 proteins.MMP3 and MMP9 levels also increased whereas aggrecan. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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