| Gene name: | SGK1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Glial cell |
| Gene ID: | 6446 |
| Category: | protein coding |
| Phenotype: | Parkinson disease |
| Experimental category: | HL |
| PMID: | 33646633 |
| Experiment: | RNA-seq//SA-β-gal activity assay//ELISA//CCK-8 assay |
| Description: | Second, our RNA‐seq data show that treating glia with an SGK1 inhibitor downregulated the pro‐oxidant Cyba, Ncf2, Recql4, Bax, and Fdxr and senescence‐associated secretory phenotype (SASP) genes. We further observed that senescence‐associated β‐galactosidase (SA‐β‐gal) activity, the hallmark senescence biomarker, was promoted in glia exposed to H2O2 and significantly rescued in the presence of the SGK1 inhibitor.In addition, in a paraquat‐induced glial senescence model, a WB analysis showed that the herbicide‐induced LMNB1 (Lamin B1) loss, a senescence‐associated biomarker, was alleviated in glial cultures treated with the SGK1 inhibitor, and levels of the pro‐senescence proteins IL6, MMP3, and p16 (CDKN2A) also decreased. Reduced secretion of IL6, a cytokine of SASP, from cultured glia treated with the SGK1 inhibitor was further demonstrated using ELISA. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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