| Gene name: | PPARA |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | EA.hy926,HUVEC |
| Gene ID: | 5465 |
| Category: | protein coding |
| Phenotype: | Atherosclerosis |
| Experimental category: | L |
| PMID: | 33728018 |
| Experiment: | SA-β-gal activity assay//Immunostainings |
| Description: | β-gal staining showed that the number of senescent cells decreased after administration of the PPARα agonist.Immunofluorescence double labeling staining showed that there were a large number of aging endothelial cells in the atherosclerotic plaque of Apoe-/- mice, and the PPARα agonist inhibited the denudation and maintained the integrity of aging endothelial cells in Apoe-/- model mice. |
| Target gene: | GDF11 |
| Official symbol(s): | GDF11 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | EMSA//Dual-Luciferase reporter assay |
| Target gene description: | The EMSA results showed that PPARα bound to the labeled target probe, and partially to the mutant probe, indicating that PPARα could bind to the GDF11 target sequence and participate in the regulation of downstream gene transcription. Double-luciferase reporter gene experiment results indicated that PPARα could bind to the GDF11-specific sequence. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...