| Gene name: | SNHG29 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HTR8/SVneo |
| Experiment: | SA-β-gal activity assay//qRT-PCR |
| Description: | SA-β-Gal increased in the SNHG29 + H2O2 cells as compared to the NC + H2O2 cells (7.8%, 15.9% and 23% respectively). We measured the levels of pro-inflammatory cytokines, such as IL-8 and TNF- α. The mRNA levels of IL-8 and TNF-α decreased in cells depleted of SNHG29 compared with NC-Si and NC-Si + H2O2 cells. However, there was an increase in the expression of IL-8 and TNF-α in cells transfected with AAV-SNHG29 compared to NC and NC + H2O2 cells. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot//SA-β-gal activity assay//qRT-PCR |
| Pathway description: | Treated cells depleted of SNHG29 (si-SNHG29 + H2O2) showed a reduction in the levels of mRNA of p53 and p21, and the protein levels of p53, phospho-p53,p21and phospho-p21, as compared to treated cells with wild-type SNHG29. Overexpression of SNHG29 in H 2O2-treated cells (SNHG29 + H2O2) showed increases in the level of mRNA of p53 and p21, and protein levels of p53, phospho-p53, p21and phospho-p21 as compared to treated cells with wild-type SNHG29. |
Annotation:
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