Aging Overview

Aging gene

Gene name: HEIH
Aging type: Prevent
Aging characteristic:
Tissue type: --
Cell name: OVCA429,OVCA433,OVCAR3
Gene ID: 100859930
Category: ncRNA
Phenotype: Ovarian cancer
Experimental category: L
PMID: 33110047
Experiment: SA-β-gal activity assay
Description: As shown in Fig. 2H, the percentage of SA-b-Gal-positive cells was increased by HEIH knockdown in OVCA429 (sh-HEIH#1: 5.2-fold higher than sh-NC group; sh-HEIH#2: 4.6-fold higher than sh-NC group) and OVCA433 cells (sh-HEIH#1: 4.8-fold higher than sh-NC group; shHEIH#2: 4.7-fold higher than sh-NC group).


Regulatory relationship

Target gene: MIR-3619-5P
Official symbol(s): MIR-3619-5P
R-AG-Target gene: Downregulation
Subcategory: Unclear
Target gene experiment: Dual-Luciferase reporter assay//qRT-PCR//RIP
Target gene description: From Figure 3D, the transfection of miR-3619-5p mimics showed no difference on the luciferase activity of pmirGLO-HEIH-Mut vectors, but significantly decreased the luciferase reporter activity of pmirGLO-HEIHWt vectors in OVCA429 (0.33-fold decreased) and OVCA433 cells (0.41-fold decreased) (n ? 3, P < 0.05). In addition, RIP assay indicated that HEIH-Wt and miR-3619-5p were apparently enriched in the Ago2 pellet but not the IgG control pellet in both OVCA429 (HEIH: 4.93-fold increased; miR-3619-5p: 3.21-fold increased) and OVCA433 cells (HEIH: 5.24-fold increased; miR-3619-5p: 3.07-fold increased). RT-qPCR analysis was carried out and the results indicated that HEIH expression was significantly decreased by miR-3619-5p mimics (OVCA429: 0.14-fold decreased; OVCA433: 0.26fold decreased), whereas HEIH knockdown markedly increased miR-3619-5p expression (OVCA429: 3.24-fold increased; OVCA433: 2.87-fold increased).

Regulatory pathway: MIR-3619-5P-CTTNBP2
R-AG-Pathway: Downregulation
Pathway experiment: Dual-Luciferase reporter assay//qRT-PCR//RIP
Pathway description: As presented in Figure 5C, the luciferase reporter activity of pmirGLO-CTTNBP2-Wt vectors was significantly reduced by miR-3619-5p mimics transfection (OVCA433: 0.38-fold decreased; OVCA429: 0.44-fold decreased). RIP assay indicated that miR-3619-5p (OVCA429: 785-fold increased; OVCA433: 833-fold increased) and CTTNBP2 expression (OVCA429: 1370-fold increased; OVCA433: 1260-fold increased) was significantly enriched in the Ago2 pellet rather than in the IgG control pellet. RT-qPCR and western blot analyses displayed that the mRNA and protein levels of CTTNBP2 were markedly decreased by miR-3619-5p overexpression (OVCA429: 0.15-fold decreased; OVCA433: 0.34-fold decreased) or HEIH knockdown (OVCA429: 0.22-fold decreased; OVCA433: 0.37-fold decreased). Additionally, the percentage of SA-b-Gal-positive cells was increased by HEIH knockdown, whereas these effects were abrogated by CTTNBP2 overexpression in OVCA429 (5.2-fold higher than sh-NC group; 0.2-fold lower than sh-HEIH#1 group) and OVCA433 cells (4.8-fold higher than sh-NC group; 0.2-fold lower than sh-HEIH#1 group).


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

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