Gene name: | HEIH |
Aging type: | Prevent |
Aging characteristic: |
Tissue type: | -- |
Cell name: | OVCA429,OVCA433,OVCAR3 |
Gene ID: | 100859930 |
Category: | ncRNA |
Phenotype: | Ovarian cancer |
Experimental category: | L |
PMID: | 33110047 |
Experiment: | SA-β-gal activity assay |
Description: | As shown in Fig. 2H, the percentage of SA-b-Gal-positive cells was increased by HEIH knockdown in OVCA429 (sh-HEIH#1: 5.2-fold higher than sh-NC group; sh-HEIH#2: 4.6-fold higher than sh-NC group) and OVCA433 cells (sh-HEIH#1: 4.8-fold higher than sh-NC group; shHEIH#2: 4.7-fold higher than sh-NC group). |
Target gene: | MIR-3619-5P |
Official symbol(s): | MIR-3619-5P |
R-AG-Target gene: | Downregulation |
Subcategory: | Unclear |
Target gene experiment: | Dual-Luciferase reporter assay//qRT-PCR//RIP |
Target gene description: | From Figure 3D, the transfection of miR-3619-5p mimics showed no difference on the luciferase activity of pmirGLO-HEIH-Mut vectors, but significantly decreased the luciferase reporter activity of pmirGLO-HEIHWt vectors in OVCA429 (0.33-fold decreased) and OVCA433 cells (0.41-fold decreased) (n ? 3, P < 0.05). In addition, RIP assay indicated that HEIH-Wt and miR-3619-5p were apparently enriched in the Ago2 pellet but not the IgG control pellet in both OVCA429 (HEIH: 4.93-fold increased; miR-3619-5p: 3.21-fold increased) and OVCA433 cells (HEIH: 5.24-fold increased; miR-3619-5p: 3.07-fold increased). RT-qPCR analysis was carried out and the results indicated that HEIH expression was significantly decreased by miR-3619-5p mimics (OVCA429: 0.14-fold decreased; OVCA433: 0.26fold decreased), whereas HEIH knockdown markedly increased miR-3619-5p expression (OVCA429: 3.24-fold increased; OVCA433: 2.87-fold increased). |
Regulatory pathway: | MIR-3619-5P-CTTNBP2 |
R-AG-Pathway: | Downregulation |
Pathway experiment: | Dual-Luciferase reporter assay//qRT-PCR//RIP |
Pathway description: | As presented in Figure 5C, the luciferase reporter activity of pmirGLO-CTTNBP2-Wt vectors was significantly reduced by miR-3619-5p mimics transfection (OVCA433: 0.38-fold decreased; OVCA429: 0.44-fold decreased). RIP assay indicated that miR-3619-5p (OVCA429: 785-fold increased; OVCA433: 833-fold increased) and CTTNBP2 expression (OVCA429: 1370-fold increased; OVCA433: 1260-fold increased) was significantly enriched in the Ago2 pellet rather than in the IgG control pellet. RT-qPCR and western blot analyses displayed that the mRNA and protein levels of CTTNBP2 were markedly decreased by miR-3619-5p overexpression (OVCA429: 0.15-fold decreased; OVCA433: 0.34-fold decreased) or HEIH knockdown (OVCA429: 0.22-fold decreased; OVCA433: 0.37-fold decreased). Additionally, the percentage of SA-b-Gal-positive cells was increased by HEIH knockdown, whereas these effects were abrogated by CTTNBP2 overexpression in OVCA429 (5.2-fold higher than sh-NC group; 0.2-fold lower than sh-HEIH#1 group) and OVCA433 cells (4.8-fold higher than sh-NC group; 0.2-fold lower than sh-HEIH#1 group). |
Annotation:
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