| Gene name: | MEG3 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | A549,MCF-7 |
| Experiment: | SA-β-gal activity assay//Western blot//qRT-PCR |
| Description: | The degree of senescence of A549 and MCF-7 cells was detected by SA-β-gal staining after depletion of lncRNA MEG3, showing a significant decrease compared with that of the control group. Next, we induced senescence by etoposide after overexpression of lncRNA MEG3 in A549 and MCF-7 cells, and increased expression of p21 and p16 was observed in senescent models.The mRNA and protein expression levels of senescence-related markers were attenuated to some extent after depletion of lncRNA MEG3 in senescent models of A549 and MCF-7 cells, indicating that lncRNA MEG3 might play an important role in the regulation of senescence induced by etoposide. |
| Target gene: | MIR-16-5P |
| Official symbol(s): | MIR-16-5P |
| R-AG-Target gene: | Inhibition |
| Subcategory: | Unclear |
| Target gene experiment: | Dual-Luciferase reporter assay |
| Target gene description: | The results showed that the luciferase activity decreased significantly in Hek293T cells co-transfected with wild type lncRNA MEG3 and miR-16-5p mimics, whereas luciferase activity was restored in cells co-transfected with mutant lncRNA MEG3 and miR-16-5p mimics, indicating that miR-16-5p is indeed a direct target gene of lncRNA MEG3 and lncRNA MEG3 plays a vital role in the senescent process by acting as a ceRNA. |
| Regulatory pathway: | MIR-16-5P-VGLL4 |
| R-AG-Pathway: | Activation |
| Pathway experiment: | qRT-PCR//SA-β-gal activity assay |
| Pathway description: | Moreover, silencing lncRNA MEG3 or VGLL4 significantly alleviated low dose etoposide induced senescence, as demonstrated by decreased p21 and p16 expression and SA-β-gal staining positive cells. Interestingly, the effect of the inhibition of lncRNA MEG3 on the senescence of tumor cells was prominently abolished by the miR-16-5p inhibitor, as suggested by increased p21 and p16 expression and SA-β-gal staining positive cells. |
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