| Gene name: | CFL1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WI-38 |
| Experiment: | SA-β-gal activity assay//Western blot//Immunofluorescence |
| Description: | Subsequently, we assessed the expression of the cell cycle inhibitors involved in senescence‐associated growth arrest. We found that the over‐expression of cofilin‐1 in young cells could induce p53, p21Cip1, p27Kip1, p16INK4, and p‐cofilin‐1, but the silencing of cofilin‐1 in senescent cells suppressed these molecules. Subsequently, we assessed the expression of the cell cycle inhibitors involved in senescence‐associated growth arrest. We found that the over‐expression of cofilin‐1 in young cells could induce p53, p21Cip1, p27Kip1, p16INK4, and p‐cofilin‐1, but the silencing of cofilin‐1 in senescent cells suppressed these molecules. We then found that the levels of SA‐β‐gal stained cells were increased by the over‐expression of cofilin‐1 in young cells and were decreased by the knockdown of cofilin‐1 in senescent cells. |
| Target gene: | TEAD1 |
| Official symbol(s): | TEAD1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR |
| Target gene description: | To investigate whether the down‐regulation of TEAD1 mRNA is associated with up‐regulated cofilin‐1 in senescent cells, we silenced cofilin‐1 and found that the TEAD1 mRNA levels were restored in senescent cells. Additionally, the over‐expression of cofilin‐1 could suppress the expression of TEAD1 transcripts and protein in H1299/tet‐on‐cofilin‐1 cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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