| Gene name: | TWIST1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Nucleus pulposus |
| Cell name: | NP Cells |
| Gene ID: | 7291 |
| Category: | protein coding |
| Phenotype: | Disc degeneration |
| Experimental category: | L |
| PMID: | 33378011 |
| Experiment: | Western blot//SA-β-gal activity assay//qRT-PCR//CCK-8 assay |
| Description: | Of note, in the TWIST overexpression group, the p53 and p21 protein were effectively suppressed compared to the TNF-α group. In addition, the collagen II and β-gal protein expression were determined by IF staining. However, TWIST1/2 overexpressed NP cells were partly efficient in inhibiting the MMP-13 and IL-1β upregulation. The cell proliferation was determined by CCK-8 assay, and the result indicated the TWIST1/2 overexpressed NP presented a higher proliferative ability than the non-transfected cells under the treatment of TNF-α. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot |
| Pathway description: | Of note, in the TWIST overexpression group, the p53 and p21 protein were effectively suppressed compared to the TNF-α group. Besides, the p53 and p21 levels were inhibited due to the supplement of TWIST protein. |
Annotation:
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