| Gene name: | MIR217 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVECs |
| Experiment: | SA-β-gal activity assay |
| Description: | The data showed that overexpression of miR-217 significantly increased the proportion of SA-β-gal positive cells in PDL12, PDL16 and PDL20 cells |
| Target gene: | SIRT1 |
| Official symbol(s): | SIRT1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | Western blot and RT-qPCR results showed that up-regulation of miR-217 in PDL8 cells significantly inhibited SIRT1 expression, whereas miR-217 inhibitor significantly inhibited SIRT1 expression in PDL44 cells. |
| Regulatory pathway: | SIRT1-P53 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | SIRT1-TP53 |
| Pathway experiment: | qRT-PCR//Western blot |
| Pathway description: | SIRT1 protein levels and mRNA levels were significantly lower in senescent PDL44 HUVECs than in young PDL8 HUVECs, while p53 protein levels and mRNA levels were significantly higher in senescent PDL44 HUVECs than in young PDL8 HUVECs |
Annotation:
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