| Gene name: | RPPH1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WI-38,IDH4 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Seven days after transfection, senescence associated β-galactosidase staining revealed that RPPH1 RNA overexpression promotes activation of acidic β-galactosidase as a marker of cellular senescence. Measurement of cell division cycle by Propidium Iodide (PI) and Flow Cytometry uncover that RPPH1 RNA overexpression arrested proliferating fibroblasts (WI-38, PDL 15) in G1 stage whereas RMRP RNA overexpression did not affect it. |
| Target gene: | MLC1 MRNA//CCR7 MRNA |
| Official symbol(s): | MLC1 MRNA//CCR7 MRNA |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//RNA-seq |
| Target gene description: | Prediction of partial complementarity was tested in MS2-tagged RNA affinity purification assay, confirming that MLC1 and CCR7 mRNAs are biochemically associated with RPPH1 RNA in proliferating fibroblasts but not senescent ones. In addition, we observed that MLC1 and CCR7 mRNAs are destabilized in senescent fibroblasts where RPPH1 RNA is less abundant in cytoplasm. We also analyzed the published RNA-seq datasets from the diverse models of senescence, and found that the levels of MLC1 and CCR7 mRNA are declined during replicative and irradiation-mediated senescence in IMR90 cells in conjunction with modestly decreased AUF1 mRNA level and increased p16 and p21 mRNA (senescent markers). Similarly, mRNAs targeted by RPPH1 RNA are unstable after AUF1 depletion in the condition when RPPH1 RNA accumulates in mitochondria. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...