| Gene name: | YAP1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | knee |
| Cell name: | SW 1353,Hs 819.T,Chondrocyte |
| Gene ID: | 10413 |
| Category: | protein coding |
| Phenotype: | Chondrosarcoma |
| Experimental category: | HL |
| PMID: | 33495462 |
| Experiment: | SA-β-gal activity assay//Atomic force microscopy imaging//EdU assay |
| Description: | YAP1 knockdown significantly increased the percentage of senescence-associated β-galactosidase (SA-β-gal)-positive cells in the two different CHS cell strains, including SW 1353 and Hs 819.T. AFM confirmed that knockdown of YAP1 increased the chondrocyte’s elastic modulus, a characteristic of senescent cells, at the single-cell level. In addition, we demonstrated that YAP1 was essential for CHS cell proliferation, as evidenced by that loss of YAP1 reduced the percentage of EdU-incorporated cells, along with the suppression of c-Myc. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//SA-β-gal activity assay |
| Target gene description: | Notably, loss of YAP1 dramatically upregulated the expression of p21 in the SW 1353 cells, regardless of cell density. As expected, overexpression of HA-YAP1-5SA upregulated the expression of its target gene Cyr61 and, reduced p21 expression and p21-induced cellular senescence in cells expressing shYAP1#4 but not SC. |
| Regulatory pathway: | YAP-P21 |
| R-AG-Pathway: | Downregulation |
| Pathway experiment: | Western blot//Immunofluorescence |
| Pathway description: | A lower concentration of VP significantly upregulated p21 expression and increased the percentage of senescent cells, while a higher concentration of VP suppressed the p21 expression, accompanied by pronounced cell apoptosis. Indeed, knockdown of p21 by sip21#3 and sip21#4 remarkably abolished the cell senescence induced by the shYAP1#3 or shYAP1#4. We also found a marked increase in DNA damage upon p21 knockdown, as seen from the representative images of the DNA tails and the calculation of the olive tail moment (OTM). In addition, we also noted that knockdown of p21 increased γ-H2AX expression, accompanied by the activation of caspase-3-dependent apoptosis. |
Annotation:
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