| Gene name: | MIF |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Nucleus pulposus |
| Cell name: | Primary Human NP Cells |
| Gene ID: | 4282 |
| Category: | protein coding |
| Phenotype: | Intervertebral disc degeneration |
| Experimental category: | L |
| PMID: | 34306312 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Western blot |
| Description: | The SA-β-Gal staining result indicated that MIF-KO did not affect the senescence rate of the NP cells, but HC loading significantly aggravated the senescence of NP cells. However, MIF-KO further exacerbated the HC loading-induced senescence of NP cells. Flow cytometry was then used to analyze the content of intracellular ROS in NP cells marked by DCFH-DA molecular probes, and the result revealed that knockout of MIF alone did not induce the accumulation of ROS. However, HC loading did increase the content of ROS in NP cells, and MIF-KO markedly exacerbated the ROS content of the HC loading-treated NP cells. In addition, the western blot analysis revealed that the expression of MIF was markedly eliminated by MIF-KO, but the expression of senescence-associated markers and oxidative stress-related marker (NT) was not affected by MIF-KO. HC loading depressed the expression of MIF compared with the control group and markedly enhanced the P53, P21, P16, and NT expression. Similarly, knockout of MIF in HC loading-treated NP cells further aggravated the expression of senescence-related and oxidative stress-related markers. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...