| Gene name: | MIR21 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | CD4+T,HEK293T |
| Experiment: | qRT-PCR//miRNA qRT-PCR//Western blot |
| Description: | In the CD4+ T cells of D-gal treated mice, the level of miR-21was significantly decreased in the hPMSC-Exo miR-21 inhibitor treatment group compared with hPMSC-Exo group. Furthermore, the miRNA level of PTEN was markedly increased, while the mRNA levels of HO-1 and NQO1 were significantly decreased after hPMSC-Exo miR-21 inhibitor treatment. In addition, the aging-related protein expression of p53 and γ-H2AX were also improved significantly in the hPMSC-Exo miR-21 inhibitor treatment group compared with hPMSC-Exo group. |
| Target gene: | PTEN |
| Official symbol(s): | PTEN |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR |
| Target gene description: | The exosomes treated with different plasmids were co-cultured with CD4+?T cells, and the expression of miR-21 and PTEN was detected. The expression of miR-21 was markedly improved in CD4+?T cells after co-cultured with hPMSC-Exo compared PBS treatment group. Conversely, hPMSC-Exo treatment greatly decreased the expression of PTEN in CD4+?T cells compared with the PBS group. Moreover, hPMSC-Exo treated with miR-21 mimics further increasing the expression of miR-21 and decreasing the expression of PTEN in CD4+?T cells compared hPMSC-Exo treatment group, while the opposite trend appeared in the hPMSC-Exo treatment miR-21inhibitor group. |
| Regulatory pathway: | PTEN/PI3K-NRF2 |
| R-AG-Pathway: | Activation |
| Pathway experiment: | Western blot |
| Pathway description: | The expression of PTEN significantly decreased in activated CD4+ T cells after cultured for 72 h, while hPMSC-Exo intervention further decreased the expression of PTEN in activated CD4+ T cells. Furthermore, the phosphorylation of PI3K and Akt were significantly increased in activated CD4+ T cells, and the hPMSC-Exo intervention further improved the phosphorylation of PI3K and Akt. In addition, we found that the expression of nuclear Nrf2 and downstream target antioxidant genes NQO1 and HO-1 was markedly improved in activated CD4+ T cells, and the hPMSC-Exo treatment further improved the expression of these proteins. Moreover, significantly improve nuclear Nrf2, NQO1, and HO-1 expression were also found in hPMSC-Exo treated CD4+ T cells after bpV(HOpic) supplementation. |
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