| Gene name: | ZKSCAN3 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Muscles of nude mice |
| Cell name: | HMSC |
| Experiment: | SA-β-gal activity assay//Clonal expansion assay//qPCR//Western blot//Ki67 staining |
| Description: | SA-β-gal staining of ZKSCAN3+/+ and ZKSCAN3-/-hMSCs (P10). Clonal expansion ability analysis of ZKSCAN3+/+ and ZKSCAN3-/- hMSCs (P10). Telomere length analysis of ZKSCAN3+/+ and ZKSCAN3-/- hMSCs (P10) by qPCR. Western blot analysis of P16, P21 in ZKSCAN3+/+ and ZKSCAN3-/- hMSCs (P10). Immunostaining of Ki67 in ZKSCAN3-/- hMSCs (P8) transduced with lentiviruses expressing luciferase (Luc) or ZKSCAN3. |
| Target gene: | LAMIN B1,LBR,KAP1,HP1α |
| Official symbol(s): | LAMIN B1,LBR,KAP1,HP1α |
| R-AG-Target gene: | Upregulation//Upregulation//Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Co-IP//Western blot |
| Target gene description: | Co-IP analysis showing that exogenous ZKSCAN3 interacts with Lamin B1, LBR, KAP1 and HP1? proteins in HEK293T cells. Western blot analysis of the expression levels of Lamin B1, LBR, KAP1 and HP1? proteins in ZKSCAN3+/+ and ZKSCAN3-/- hMSCs (P10). |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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