| Gene name: | SIRT7 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HMSC |
| Experiment: | SA-β-gal activity assay//Clonal expansion assay//RT-qPCR//Western blot |
| Description: | We also observed increased percentages of senescence-associated β-galactosidase (SA-βgal)-positive cells, compromised clonal expansion ability, decreased ratios of Ki67-positive cells and reduced percentages of S-phase cells in SIRT7?/? relative to SIRT7+/+ hMSCs. Consistent with the accelerated senescence phenotypes, we observed the upregulation of cyclin-dependent kinase inhibitors and aging markers CDKN1A (P21) and CDKN2A (P16) at both mRNA and protein levels, along with transcriptional downregulation of LMNB1 and TMPO in SIRT7?/? hMSCs. |
| Target gene: | LINE1 |
| Official symbol(s): | LINE1 |
| R-AG-Target gene: | Inhibition |
| Subcategory: | Unclear |
| Target gene experiment: | ChIP-qPCR |
| Target gene description: | Using ChIP-qPCR assay, we detected that SIRT7 bound specific LINE1 promoter regions in SIRT7+/+ but not in SIRT7?/? hMSCs. |
| Regulatory pathway: | CGAS-STING |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | CGAS-STING1 |
| Pathway experiment: | Western blot |
| Pathway description: | Activation of the cGAS-STING pathway was evident in SIRT7deficient hMSCs, as detected by increased levels of phosphorylated forms of NF-κB P65, TANK-binding kinase 1 (TBK1) and IFN regulatory factor 3 (IRF3). |
Annotation:
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