| Gene name: | PIK3R3 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | LoVo,SW48 |
| Gene ID: | 8503 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | L |
| PMID: | 32973127 |
| Experiment: | SA-β-gal activity assay//Western blot//CCK-8 assay//flow cytometry |
| Description: | WB of PIK3R3 and p21 both in LoVo and SW48 cells transfected with siNC, siPIK3R3#1, and siPIK3R3#2. CCK8 assays of LoVo and SW48 cells transfected with PIK3R3-specific siRNA. Cell cycle distribution of LoVo and SW48 cells transfected with siNC, siPIK3R3#1, and siPIK3R3#2 was measured byflow cytometry. SA-β-gal staining of PIK3R3-knockdown LoVo or SW48 cells after treatment of Dox (0.25μM, 48 h). |
| Target gene: | P53 |
| Official symbol(s): | P53 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | IP |
| Target gene description: | Immunoprecipitation assays were performed for LoVo cells with antiPIK3R3 or anti-p53 antibodies. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | CHIP//Western blot |
| Pathway description: | ChIP assays of p53 binding to the CDKN1A promoter region in LoVo transfected with PIK3R3-expressing plasmid. WB of PIK3R3, p53, and p21 in LoVo and SW48 cells transfected with PIK3R3 plasmid or/and His-p53 plamid. |
Annotation:
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