| Gene name: | MIR29A |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HMSC |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | Senescence-associated-β-galactosidase (SA-β-gal) staining. Compared with the siGFP-transfected group, siDGCR8-transfected hMSCs showed increased numbers of The percentage of SA-β-gal-positive cells was significantly higher in siDGCR8-transfected hMSCs. Western blot showing the protein levels of tumour suppressors and senescence markers, including p21, p53, and p-p53. |
| Target gene: | DNMT3A |
| Official symbol(s): | DNMT3A |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Methylation |
| Target gene experiment: | Luciferase reporter assay//RT-qPCR//Western blot |
| Target gene description: | 293T cells were co-transfected with luciferase reporters carrying either the wild-type DNMT3A 3′ UTR (Dnmt3a) or the mutagenised DNMT3A 3′ UTR (mDnmt3a), as well as 50 nM negative control mimic (miR-Control) or miR-29a-3p or/and miR-30c-5p mimics. The mRNA levels of DNMTs were measured by quantitative real-time PCR. Immunoblot showing DNMT3A protein levels in DGCR8 knockdown cells with or without miR-29a-3p or/and miR-30c-5p forced expression (bottom). |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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