| Gene name: | CD276 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HCT116,RKO |
| Gene ID: | 80381 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | HL |
| PMID: | 33958586 |
| Experiment: | SA-β-gal activity assay//Western blot//CCK-8 assay//Colony formation assay//Flow cytometry |
| Description: | Western blot analysis of p21 and p53 in sh-NC cells or shB7-H3 CRC cells treated with DOX. SA-β-Gal activity of sh-NC cells or shB7-H3 CRC cells treated with DOX was examined. Cell viability in sh-NC cells or shB7-H3 CRC cells treated with DOX after 24, 36, 48, and 96 h was examined by CCK-8 assays. The colony formation of shNC cells or shB7-H3 CRC cells treated with DOX was examined. Cell cycle analysis by PI staining in sh-NC cells or shB7-H3 CRC cells with DOX treatment was examined through flow cytometry. |
| Target gene: | TM4SF1 |
| Official symbol(s): | TM4SF1 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | RT-qPCR//Western blot |
| Target gene description: | The mRNA expression of TM4SF1 in EV cells or B7-H3 CRC cells treated with DOX. Western blot analysis of TM4SF1 and p21 in sh-NC cells or shB7-H3 CRC cells treated with DOX. Western blot analysis of TM4SF1 and p21 in EV cells or B7-H3 CRC cells treated with DOX. |
| Regulatory pathway: | AKT-TM4SF1-SIRT1 |
| R-AG-Pathway: | Upregulation |
| Pathway experiment: | Western blot |
| Pathway description: | The western blot results showed that B7-H3 knockdown decreased SIRT1 protein expression, while TM4SF1 overexpression reversed the effect of B7-H3 knockdown on SIRT1 expression in low-dose DOX induced senescent HCT116 and RKO cells. Moreover, B7-H3 overexpression increased SIRT1 protein expression, while TM4SF1 knockdown abolished the effect of B7-H3 overexpression on SIRT1 expression in low-dose DOX-induced senescent CRC cells. |
Annotation:
Loading,please wait...