| Gene name: | AHNAK |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | BJ cells |
| Experiment: | SA-β-gal activity assay |
| Description: | To induce senescence by Nutlin-3, BJ cells were seeded at low density, and then transfected with indicated siRNA. Subsequently, cells were left treated with DMSO or Nutlin-3 (20 mM) for indicated days, and SA-b galactosidase assay is performed as described previously. |
| Target gene: | 53BP1 |
| Official symbol(s): | TP53BP1 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | IP//Western blot |
| Target gene description: | Western blot (WB) using anti-mCherry and anti-AHNAK antibodies after immunoprecipitation (IP) with IgG or Anti-AHNAK beads as indicated. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | Immunoblot analysis of BJ fibroblast following transfection with control or indicated siRNA. Cell lysates prepared from untreated and NCS-treated cells were analyzed using immunoblot using the specified antibodies. |
Annotation:
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