| Gene name: | CRY1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | UMUC3 Res cells,EJ Res cells |
| Gene ID: | 1407 |
| Category: | protein coding |
| Phenotype: | Bladder cancer |
| Experimental category: | L |
| PMID: | 33650658 |
| Experiment: | SA-β-gal activity assay//Western blot//qPCR |
| Description: | After CRY1 knockdown, p21 mRNA expression was significantly downregulated, but its protein expression was not significantly changed in both Res cells. The p53 mRNA expression did not change significantly in EJ Res cells, but was significantly increased in UMUC3 Res cells. Moreover, its protein expres- sion was significantly enhanced after CRY1 knockdown in both cell lines. The si-CRY1-1 demonstrated a higher efficiency compared with the si CRY1 2 to inhibit CRY1 expression. Therefore, the si-CRY1-1 was used in subsequent experiments. It was identified that CRY1 knock- down induced apparent senescence in the PTX-treated Res cells. |
| Target gene: | P53 |
| Official symbol(s): | P53 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//qPCR |
| Target gene description: | The p53 mRNA expression did not change significantly in EJ Res cells, but was significantly increased in UMUC3 Res cells. Moreover, its protein expres- sion was significantly enhanced after CRY1 knockdown in both cell lines. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...