| Gene name: | CXCR2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | LL2 cells,H460 cells |
| Gene ID: | 3579 |
| Category: | protein coding |
| Phenotype: | Lung cancer |
| Experimental category: | HL |
| PMID: | 33814009 |
| Experiment: | CCK-8 assay//SA-β-gal activity assay//qRT-PCR//Western blot |
| Description: | Selective inhibitor of CXCR2, SB225002, was confirmed to inhibit the proliferation of lung cancer cells in a both time-dependent and dose-dependent manner. To investigate the role of CXCR2 in tumor cell senescence, the expres- sion of SA-β-gal was detected while using SB225002 on LL2 and H460 cells. After 24 h, the result showed that the percentage of β-gal-positive cells in SB225002 group was higher than that of control group. The senescence-associated markers p16 and p21 were up- regulated after using SB225002 and down-regulated after using CXCL2. |
| Regulatory pathway: | P38-ERK MAPK |
| R-AG-Pathway: | Activation |
| Pathway experiment: | Western blot |
| Pathway description: | After using SB225002 and CXCL2 on LL2 cells, p38/ERK MAPK signaling pathway was found involved in CXCR2-associated signaling pathway. The levels of p38 and ERK proteins phosphorylation were obviously al- tered, whereas p-JNK almost remained unchanged during activation or inhibition of CXCR2. |
Annotation:
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