| Gene name: | TNF |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | A375,HT29 |
| Experiment: | SA-β-gal activity assay |
| Description: | Characteristic enlarged senescent cells containing blue-dyed precipitates indicating increased senescence-associated β- galactosidase activity appeared in both melanoma lines A375hTNFa and M4BeuhTNFa. Senescence induction was observed also in parental melanoma cells treated with recombinant human TNFα protein. In colorectal carcinoma cells HT29hTNFa and HCT116h TNFa, no differences in the incidence of senescent cells were observed. HCT116 parental cells entered senescence spontaneously and more frequently in comparison to the HT29 cells. |
| Target gene: | TRAIL//IL6 |
| Official symbol(s): | TRAIL//IL6 |
| R-AG-Target gene: | Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR |
| Target gene description: | CSCs- like markers remained unchanged In engineered melanoma cells A375hTNFa, significant overexpression of inflammatory cytokine IL6 was in- duced. Overexpression of TNFα in cells HT29hTNFa significantly stimulated expression of apoptosis-inducing ligand TRAIL. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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