| Gene name: | TBK1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | NPCs |
| Gene ID: | 29110 |
| Category: | protein coding |
| Phenotype: | Intervertebral disc degeneration |
| Experimental category: | L |
| PMID: | 34026282 |
| Experiment: | Western blot//SA-β-gal activity assay//TUNEL assay |
| Description: | We markedly found that cells transfected with lentivirus-TBK1 (Lv- TBK1) remarkably exhibited less p16INK4a (p16) activity than cell transfected with lentivirus-Control (Lv-Ctrl) at passage 15, show- ing that TBK1 exerted an enormous function on NPCs senescence. The p16 and cleaved caspase-3 (CC3) expression levels were assessed to evaluate the TBK1-mediated anti-apoptotic and anti- senescence. Therefore, the substantial results in our study showed that TNF-a significantly increased in the NPCs and the expressions level of p16 and CC3, suggesting that TNF-a induced apoptosis and premature senescence, which were inhibited by Lv-TBK1. From the TUNEL staining and SA-b-gal staining, we could observe that TNF-a stimulation markedly increased the positive points of TUNEL and SA-b gal. Conversely, administration of Lv- TBK1 significantly reversed the TNF-1a induced excessive senes- cence and apoptosis. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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