| Gene name: | PRKG1-AS1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Skeletal muscle |
| Cell name: | Myoblasts |
| Experiment: | Cell viability assay//Flow cytometry//qRT-PCR//Western blot//CCK-8 assay |
| Description: | CCK-8 assay showed that knock-down of PRKG1-AS1 could significantly increase cell viability in a time-dependent manner. Besides, flow cytometry demonstrated that cell apoptosis was significantly decreased by knocking-down of PRKG1-AS1 compared with the model group. In order to validate the effect of PKG1-AS1 at molecular level, we detected the mRNA and protein expression of muscle regulatory factors, including MyoD, MyoG, Myf2c and Myf5. These four factors were significantly decreased in the dexamethasone-induced muscle atrophy cell model (P < 0.05). After transfection with si-PRKG1-AS1, their expression was significantly upregulated both at mRNA level (P < 0.05). Western blot analysis showed consistent results with qRT-PCR. |
| Target gene: | MYOD//MYOG//MEF2C |
| Official symbol(s): | MYOD//MYOG//MEF2C |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | In order to validate the effect of PKG1-AS1 at molecular level, we detected the mRNA and protein expression of muscle regulatory factors, including MyoD, MyoG, Myf2c and Myf5. These four factors were significantly decreased in the dexamethasone-induced muscle atrophy cell model (P < 0.05). After transfection with si-PRKG1-AS1, their expression was significantly upregulated both at mRNA level (P < 0.05). Western blot analysis showed consistent results with qRT-PCR. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...