| Gene name: | HNRNPF |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | hMSCs |
| Experiment: | SA-β-gal activity assay//Cell cycle analysis//Colony formation assay//CCK-8 assay//Immunofluorescence |
| Description: | Knockdown of hnRNP F in 5PDs of hMSCs largely accelerated cell senes- cence, which indicated by the dramatic increase of SA-β-gal activity.More severe retardation of cell proliferation was observed after knockdown of hnRNP H1 in hnRNP F KD HeLa cells. Furthermore, knockdown of both hnRNP H1 and H2 in hnRNP F KD HeLa cells further reduced cell proliferation, indicating an additive effect of hnRNP F, H1, and H2 on regulating cancer cell proliferation. We also found that hnRNP F depletion caused significant G2/M accumulation and triple knockdown of hnRNP F, H1, and H2 triggered more obvious G2/M arrest.Similar to HeLa cells, hnRNP F deletion significantly reduced hMSCs telomerase activity ,drastically inhibited hMSCs proliferation and Ki67-positive cell numbers , and caused significant hMSCs G2/M cell cycle arrest.HnRNP F or H1 overexpression in 12PDs of hMSCs remarkably retarded cell senescence . HnRNP F or H1 overexpression also significantly elevated Ki67-positive cell numbers , indicating that enforced expression of hnRNP F/H preserve hMSCs proliferation capacity. |
| Target gene: | TCAB1//HTERC |
| Official symbol(s): | TCAB1//HTERC |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Immunofluorescence//Biotinylated RNA pull down assay |
| Target gene description: | The immunofluorescent signals of FLAG-hTERT overlapped with a specific Cajal bodies marker Coilin signals in the nuclei in both HeLa and U2OS control cells.The results showed that hnRNP F/H may form stable ribonucleoprotein complex with hTERC through direct binding. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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