| Gene name: | PIM1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Cardiomyocytes |
| Experiment: | RT-PCR//Fluorescence quenching assay |
| Description: | PIM1 cells contained 1.55- fold longer telomeres than GFP cells after three passages as revealed by quantitative RT-PCR.Telomeres were 27% longer in PIM1 myocardium and 31% shorter in Pim1-KO hearts compared to NTg as revealed by qRT–PCR.Telomere fluorescence intensity was 28% greater in PIM1 cardiomyocytes and reduced by 30% in Pim1-KO cardiomyocytes compared to NTg controls. |
| Regulatory pathway: | TGFβ |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | TGFB1 |
| Pathway experiment: | Western blot//qRT-PCR//Immunoblotting(孟丽加的) |
| Pathway description: | In the presence of TGFb1 agonist, PIM1 exhibited a 41% reduction in pSMAD2 levels compared to GFP as evaluated by immunoblotting. Inhibitory effects of PIM1 on TGFb signalling were confirmed by expression of canonical downstream target SERPINE1 and non-canonical target N-CADHERIN as measured via qRT-PCR. PIM1 displayed a 64% and 42% reduction in mRNA expression of SERPINE1 and N-CADHERIN, respectively, compared to GFP.Cardiac-specific overexpression of PIM1 resulted in a 39% reduction in pSmad2 levels compared to NTg.Pim1-KO cardiomyocytes also displayed an up-regulation of pSmad2 compared to PIM1 Immunoblot analysis of pSmad3 also revealed a reduction of TGFβ signalling in PIM1 compared to NTg and Pim1-KO. |
Annotation:
Loading,please wait...