| Gene name: | MIR34C |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | KG-1a |
| Experiment: | SA-β-gal activity assay//Western blot//Flow cytometry//qRT-PCR//EdU assay |
| Description: | Forced expression of miR-34c-5p resulted in a decreased cell number, proliferation index, and percentage of EdU+ cells.. Increased miR-34c5p induced an increase in the number of G0/G1 phase cells and decreased cell cycle-associated protein levels.Up-regulation of miR-34c-5p failed to induce apoptosis but resulted in significantly increased senescence in KG-1a cells . Furthermore , two typical senescence-associated secretory factors , IL-6 and plasminogen activator inhibitor-1 (PAI-1), were elevated under treatment with miR-34c-5p mimic at 48 h postelectrotransfection . |
| Target gene: | RAB27B |
| Official symbol(s): | RAB27B |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | qPCR//Western blot//Luciferase reporter assay |
| Target gene description: | Using qPCR and Western blotting, we further confirmed that RAB27B mRNA and protein levels were decreased in 34cOE-KG-1a cells.We selected one of the most likely binding sites according to the folding energy and P- value parameters provided by rna22 and performed a luciferase reporter assay. We found that co-transfection of the luciferase reporter and miR-34c-5p mimic into KG-1a cells produced lower luciferase activity than cells co-transfected with miR-NC, but miR-34c-5p mimic did not reduce luciferase activity with the mutated RAB27B 3′-UTR, indicating that miR-34c-5p is a specific regulator of RAB27B. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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