| Gene name: | MIR34A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | A549,BEAS-2B |
| Gene ID: | 407040 |
| Category: | ncRNA |
| Phenotype: | Idiopathic pulmonary fibrosis |
| Experimental category: | L |
| PMID: | 27979858 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Western blot//qRT-PCR//BrdU assay |
| Description: | MiR-34a was potent in inducing senescence of human lung epithelial A549 cells, as indicated by elevated senescence-associated β-galactosidase activity and increased expression of senescence markers p21 and PAI-1 in miR-34a transfected lung epithelial cells. Consistent with this senescent phenotype, miR-34a transfected lung epithelial cells also demonstrated decreased proliferation. The prosenescence activity of miR-34a was also confirmed in human lung epithelial cell line BEAS-2B. |
| Target gene: | SIRT1 |
| Official symbol(s): | SIRT1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR |
| Target gene description: | We confirmed that Sirt1 expression was decreased in lung epithelial cells that were transfected with miR-34a. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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